Influence of the Modulation of the Protein Corona on Gene Expression Using Polyethylenimine (PEI) Polyplexes as Delivery Vehicle

The protein corona can significantly modulate the physicochemical properties and gene delivery of polyethylenimine (PEI)/DNA complexes (polyplexes). effects on transfection have been well studied in terms averaged expression a whole cell population. Such evaluation methods give excellent reliable statistics, but they general provide final efficiency without reflecting dynamic process expression. In this regard influence bovine serum albumin (BSA) PEI polyplexes also single level via live imaging is analyzed. results reveal that although BSA causes difference overall mRNA transcription, behavior individual similar, including mitosis-dependent expression, distributions onset time, pattern two daughter cells, kinetics successfully transfected cells. Comparison ensemble data cultures indicate does not alter after nuclear entry, division, polyplex dissociation, Its other steps vitro before entry shall render transfection.